4/14/2021 0 Comments Concentration And Dilution Lab
On the Insert tab, click on the Scatter icon and select Scatter with Straight Lines and Markers from its drop-down menu to generate the standard curve.The concentration factor is the initial volume divided by the final solution volume; the dilution factor would be the inverse of the concentration factor.
For example, if you take 1 part of a sample and add 9 parts of water (solvent), then you have made a 1:10 dilution; this is 110th (0.1) of the concentration of the original solution and has a dilution factor of 10. Concentration And Dilution Lab Serial Dilutions AreThese serial dilutions are often used to determine the approximate concentration of an enzyme (or molecule) to be quantified in an assay. Serial dilutions allow for small aliquots to be diluted instead of wasting large quantities of materials, are cost-effective, and are easy to prepare. To determine the concentration at each step of the series, you divide the previous concentration by the dilution factor. In the diagram, indicate the volume being withdrawn from the concentrated solution, the volume of water added, the concentration of the new solution, and the total volume. This five-fold serial dilution would have concentrations of 100, in first diluted tube, in second diluted tube, in third diluted tube. Use a dry clean paper towel to wipe off any fingerprints on the bottom of the plate. But make sure that you released all liquid into the first well. Pipet 50 L of the original blue dye into the first well (B1). Then make sure that you released all liquid into the first well. Pipet 40 L of the original blue dye into the first well (C1). Does that save time compared to working with individual cuvettes and a spectrophotometer We will use a microplate with 96 wells, so that you can perform all of your serial dilutions onto one plate and scan the entire plate with the microplate reader once. Using blue dye, you will make a 1:2 serial dilution on row A, make a 1:4 serial dilution on row B, and a 1:5 serial dilution on row C. Take a photo of the computer screen and note the Absorbance data in the tables below. The standard curves are most often used to determine the concentration of unknown samples by comparing them to reference samples with known concentrations. Later in the course, we will use standard curves to measure amounts of extracted protein and to determine the size of DNA molecules. In todays lab, you made three serial dilutions and should be able to calculate the concentrations for each dilution. Using Excel, you will prepare standard curves for each serial dilution and determine if your standard curve is accurate. Then you will determine the concentration of unknown samples, using your standard curves. For the standard curve, this value measures how strong the linear relationship is between the reagent concentration (X-axis) and the absorbance value (Y-axis). If the R2 value 1, then that shows a perfect positive relationship.
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